Seventh-seeded Shenandoah (9-9) is looking for its sixth semi-final round appearance in seven seasons of league play.
In 2015, as the second seed, Shenandoah downed seventh-seeded R-MC 1-0 thanks to an Alysha McCleaf goal late in the second half.
The 'Jackets won the regular season meeting this season, 4-0, on October 10 in Ashland.
The roles are reversed Wednesday as the Yellow Jackets host as the No. 2 seed and SU travels as the No. 7.
The Yellow Jackets lead the nation in goals against average at 0.14 per game – R-MC has allowed just two goals all season long with Washington and Lee tallying once in a 1-1 tie on September 22 and Bridgewater netting the game's only goal in a 1-0 victory on October 20.
Freshman Nicole Czyzewski leads the 'Jackets in scoring with nine goals and three assists for 21 points with sophomore Casey Kent adding six goals and four assists for 16 points.
Edye Carr has played the majority of minutes, 812, in the goal and has 32 saves, while Christina Newman has played 405 minutes without allowing a goal. Newman has been forced to make just three saves in recording seven shutouts.
Junior Emily Yergin leads Shenandoah in scoring with 16 goals and 11 assists for 43 points. She is second in the league in all three categories.
In the goal, freshman Emilie Smith has started 12 of 13 games she has appeared in and has six of the Hornets nine wins.
Smith has made 43 stops this season.
Randolph-Macon is the No. 2 scoring team in the league at 3.43 goals per game with SU fourth at 2.83.
Shenandoah gives up 2.51 goals per outing.