Shenandoah (15-6-1) claims the league's automatic bid into the NCAA Tournament with the win over CNU (11-8-1).
The contest is also the final one that the team will play in the USA South. Next season, the Hornets join the Old Dominion Athletic Conference.
The announcement of the NCAA Tournament field will be made Monday on www.ncaa.com with the first round of play scheduled for this coming weekend.
Junior Amber Womack tallied the only goal of the game as she scored unassisted in the 70th minute.
Womack knocked home a rebound of a deflected shot to score what proved to be the game-winning tally.
Christopher Newport had three shots in the remaining 20 minutes, two of which were on frame, but SU keeper Kat Terza posted two of her three saves to keep her sheet clean.
Terza, who allowed two goals in 290 minutes of action in the tournament, was named as the tournament MVP.
CNU outshot Shenandoah 10-5 in the contest but Shenandoah had four of the game's five corners.
The win is also the 50th of coach Liz Pike's SU career.